Peptides are typically sold as dry lyophilized powders, which makes them shelf-stable and safe for storage. However, in order to use these peptides, they must be reconstituted into liquid form. The reconstituted peptides can then be injected and used in experiments.
In the process of reconstitution, there are a few important things to keep in mind. First, the peptide should be dissolved in a solvent that is safe for injection and is appropriate for the experiment being performed. Next, it is important to carefully measure the volume of the peptide needed. Finally, the reconstituted peptide should be stored properly to ensure that it does not degrade over time.
The most common solvent for peptide reconstitution is bacteriostatic water. This solution is designed to kill bacteria that may be present in the peptide. However, there are other options as well, depending on the specific peptide and the experiment being conducted. For instance, some peptides dissolve better in a buffer solution than others.
When adding the peptide to the bacteriostatic water, it is crucial to do so slowly and to avoid spraying the solution or vigorously shaking the vial. This can ruin the peptide, as well as damage the needle and syringe. Instead, it is best to drip the peptide in and very gently swirl the vial. This method also helps to prevent adsorption, which can occur when a peptide is exposed to a non-sterile surface and can dramatically distort subsequent results. peptide vials